Mississippi Cultural Crossroads is housed in two historic buildings in downtown Port Gibson, Mississippi. The town sits astride the Great River Road, Highway 61, where it crosses the south fork of Bayou Pierre.

This historic town was founded in 1803 by Samuel Gibson. It was the scene of an important Civil War battle in 1863, when General Grant crossed the Mississippi River just below Port Gibson in his successful effort to seize the city of Vicksburg and cut the Confederacy in two. Many antebellum homes remain along Church Street because, according to local legend, Grant declared the town "too beautiful to burn."

In the 1960s and 70s it became the scene of an important struggle in the Civil Rights Movement when the local chapter of the NAACP initiated a boycott of white merchants to back up their demands for political and civic equality. A damage suit against the boycotters by local merchants was eventually settled in favor of the NAACP by the United States Supreme Court.

Port Gibson is just minutes off the Natchez Trace Parkway, approximately halfway between Jackson and Natchez. (Take the exit at mile marker 37 or 41.) It is 30 miles south of Vicksburg on U.S. 61, and 65 miles southwest of Jackson on Mississippi 18. U.S. 61 becomes Church Street in Port Gibson, and Mississippi Cultural Crossroads is at 507 Market Street (also known as Main Street), two blocks west of Church. MCC is at the intersection of Market and Fair Streets, one block south of the Claiborne County Courthouse and immediately across the street from the Claiborne County Administration Building.

Mississippi Cultural Crossroads is normally open from 8 a.m. to 5 p.m. Monday-Friday. It is normally closed on weekends, but special arrangements can be made by calling ahead at 601-437-8905.